HPLC

 
     
 

Liquid chromatography (LC) is common technique to separate mixtures of components by using of a liquid mobile phase. The most popular category of liquid chromatography is high-performance liquid chromatography (HPLC). The method employs a mobile phase, usually a solvent (or a mix of solvents which is forced through a tightly packed column by use of a pump. Sample is injected into the column and transports by the high pressurized solvent flow to a detector. The separation is based on swiping velocities of analytes in the column that are affected by the degree of interaction of each compounds with the stationary phase in column. The detector is placed at the end of the column and monitors separated components.

 
   

 

 

 

 
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